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human pd 1 fc chimera protein  (R&D Systems)


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    R&D Systems human pd 1 fc chimera protein
    Human Pd 1 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a – c , Workflow ( a ), representative TIRF images ( b ), and quantification results ( c ) of the number of signaling molecules per TCR following introduction of PD-1–PD-L1 inhibitory signaling in T cells. d , Stoichiometry of the TCR signalosome under PD-1–PD-L1 inhibitory signaling. e , Quantified sensitivity of signaling molecules within the TCR signalosome to PD-1–PD-L1 inhibitory signaling. f , Generation of T cells expressing low or high levels of PD-1. g , h , Representative TIRF images ( g ) and corresponding quantification results ( h ) of the number of signaling molecules per TCR in T cells expressing low or high levels of PD-1, with or without PD-1–PD-L1 inhibitory signaling. Data in f are presented as mean ± SD; data in c , d , and h are presented as mean ± SEM. In c , d , and h , ≥30 individual cells were analyzed per condition. Statistical significance was assessed using an unpaired two-tailed Student’s t -test (*** P ≤ 0.001). Data in c , d , and h are representative of two replicates using T cells from two blood donors. Data in f are representative of three replicates using T cells from three blood donors.

    Journal: bioRxiv

    Article Title: Quantitative extrapolation from single-tags (QuEST) immunofluorescence microscopy to derive TCR signalosome stoichiometries in human primary T cells

    doi: 10.64898/2026.03.28.715001

    Figure Lengend Snippet: a – c , Workflow ( a ), representative TIRF images ( b ), and quantification results ( c ) of the number of signaling molecules per TCR following introduction of PD-1–PD-L1 inhibitory signaling in T cells. d , Stoichiometry of the TCR signalosome under PD-1–PD-L1 inhibitory signaling. e , Quantified sensitivity of signaling molecules within the TCR signalosome to PD-1–PD-L1 inhibitory signaling. f , Generation of T cells expressing low or high levels of PD-1. g , h , Representative TIRF images ( g ) and corresponding quantification results ( h ) of the number of signaling molecules per TCR in T cells expressing low or high levels of PD-1, with or without PD-1–PD-L1 inhibitory signaling. Data in f are presented as mean ± SD; data in c , d , and h are presented as mean ± SEM. In c , d , and h , ≥30 individual cells were analyzed per condition. Statistical significance was assessed using an unpaired two-tailed Student’s t -test (*** P ≤ 0.001). Data in c , d , and h are representative of two replicates using T cells from two blood donors. Data in f are representative of three replicates using T cells from three blood donors.

    Article Snippet: Unlabelled primary antibodies : anti-human CD8α antibody (Cell Signaling Technology, Cat#85336), anti-human CD28 antibody (Cell Signaling Technology, Cat#38774S), anti-human CD45 antibody (Cell Signaling Technology, Cat#13917S), anti-human PD-1 antibody (Cell Signaling Technology, Cat#86163T), anti-human Lck antibody (Cell Signaling Technology, Cat#2787S), anti-human ZAP-70 antibody (Cell Signaling Technology, Cat#3165S), anti-human LAT antibody (Cell Signaling Technology, Cat#45533S), anti-human PLCγ1 antibody (Cell Signaling Technology, Cat#5690S), and anti-human phospho-ZAP-70 (Tyr319) antibody (Cell Signaling Technology, Cat#2701).

    Techniques: Expressing, Two Tailed Test